THE INFLUENCE OF SEMEN CRYOPRESERVATION ON THE RELEASE OF SOME ENZYMES FROM SIBERIAN STURGEON (ACIPENSER BAERII) AND STERLET (ACIPENSER RUTHENUS) SPERMATOZOA
Beata Sarosiek*, Andrzej Ciereszko**, Adam Rzemieniecki***, Józef Domagała***, Jan Glogowski*,****
*Molecular Andrology Group, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences Olsztyn, Poland
**Semen Biology Group, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences Olsztyn, Poland
***Department of General Zoology, Szczecin University, Poland
****Division of Evolutionary Genetics, University of Warmia and Mazury in Olsztyn, Poland
ABSTRACT. The milt of individual males of Siberian sturgeon (Acipenser baerii) and sterlet (Acipenser ruthenus) was frozen without cryoprotector (at –79°C) or cryopreserved with methanol as the cryoprotector. The activity of arylsulfatase (AS), acid phosphatase (AcP), b-N-acetylglucosaminidase (NAGase), and protein concentration was determined. The protein concentration and enzymatic activities in supernatant obtained after cryopreservation were higher than in milt plasma, but they were lower than that in the material obtained after freezing at –79°C. The protein and enzymatic leakage of sterlet spermatozoa was statistically higher in supernatants that had been frozen at –79°C than in those that had undergone cryopreservation. Differences in the protein and AS leakage the Siberian sturgeon supernatants were also observed.
Key words: SIBERIAN STURGEON (ACIPENSER BAERII), STERLET (ACIPENSER RUTHENUS), SEMEN, CRYOPRESERVATION
CORRESPONDING AUTHOR:
Dr Beata Sarosiek, Polska Akademia Nauk, Instytut Rozrodu Zwierząt i Badań Żywności, Zakład Andrologii Molekularnej,
ul. Bydgoska 1/8, 10-243 Olsztyn, tel./fax: +48 89 5357421;
e-mail:pirosia@pan.olsztyn.pl