VERIFICATION OF MEIOTIC GYNOGENESIS IN BROOK TROUT, SALVELINUS FONTINALIS (MITCHILL) USING MICROSATELLITE DNA MARKERS
Dorota Fopp-Bayat*, Małgorzata Jankun*, Henryk Kuźmiński**
*Department of Ichthyology, Faculty of Environmental Sciences and Fisheries,
University of Warmia and Mazury in Olsztyn, Poland
**The Stanisław Sakowicz Inland Fisheries Institute, Salmonid Research Department in Rutki, Poland
ABSTRACT. The microsatellite DNA technique was applied to assess rapidly the overall success of treatments designed to induce gynogenesis. The results of gynogenesis were verified based on the analysis of two microsatellite loci (Sfo-296 and Sfo-262). Three brook trout, Salvelinus fontinalis (Mitchill), females, one brown trout, Salmo trutta morpha fario L., male, and their offspring (30 individuals from each female) were studied. Locus Sfo-292 was monomorphic in the studied brook trout offspring while Sfo-262 was polymorphic. The paternal genotype was absent in the studied gynogenotes. This simple microsatellite-based technique could be generally applicable for the verification of gynogenesis in brook trout when the UV-irradiated milt of brown trout is used.
Key words: BROOK TROUT, GENETIC MARKERS, GYNOGENESIS, MICROSATELLITE DNA, SALVELINUS FONTINALIS
CORRESPONDING AUTHOR:
Dorota Fopp-Bayat, Uniwersytet Warmińsko-Mazurski, Wydział Ochrony Środowiska i Rybactwa, Katedra Ichtiologii, ul. Oczapowskiego 5, 10-718 Olsztyn; Tel./Fax: +48 89 5234772, +48 89 5233754, e-mail: foppik@uwm.edu.pl