Archives of Polish Fisheries

Vol. 16 Fasc. 2

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Arch. Pol. Fish.     Vol. 16 Fasc. 2            2008 p. 179-188

QUALITY PARAMETERS AND SELECTED BIOCHEMICAL MARKERS OF ASP, ASPIUS ASPIUS (L.), SEMEN OBTAINED AFTER HORMONAL STIMULATION WITH OVAPRIM OR OVOPEL

Beata Irena Cejko*, Dariusz Kucharczyk**, Katarzyna Targońska**, Dorota Kubiak*, Beata Sarosiek*, Jan Glogowski *,***

*Departament of Molecular Andrology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences in Olsztyn, Poland
**Chair of Lake and River Fisheries, Faculty of Environmental Sciences and Fisheries, University of Warmia and Mazury in Olsztyn, Poland
***Chair of Ichthyology, Faculty of Environmental Sciences and Fisheries, University of Warmia and Mazury in Olsztyn, Poland

ABSTRACT. The aim of the study was to determine the basic quality parameters and selected biochemical markers of asp, Aspius aspius (L.), semen after spermiation was stimulated with Ovaprim and Ovopel. Sperm motility and concentration, osmotic pressure, total protein content, and activities of acid phosphatase (AcP), lactate dehydrogenase (LDH), and b-N-acetylglucosaminidase (b-NAG) were determined. It was revealed that higher sperm motility and concentration and higher seminal plasma protein content were obtained after stimulation with Ovaprim. The osmotic pressure of the seminal plasma estimated for males following the administration of Ovopel was higher than after they had been treated with Ovaprim. It was determined that enzyme activity in the seminal plasma of the fish stimulated with Ovaprim was higher in comparison with results obtained after they had been treated with Ovopel. Significant, positive dependencies were confirmed between the concentration of sperm and the total protein content in the seminal plasma (r2 = 0.492) and the activity of b-NAG (r2 = 0.779); among the total protein content in the seminal plasma and the activities of AcP (r2 = 0.476), LDH (r2 = 0.564), and b-NAG (r2 = 0.738); and between the activities of AcP and LDH (r2 = 0.483) and between the activities of LDH and b-NAG (r2 = 0.844).

Key words: ASP, SEMEN, SEMINAL PLASMA, OVAPRIM, OVOPEL

CORRESPONDING AUTHOR:

Beata I. Cejko, Instytut Rozrodu Zwierząt i Badań Żywności PAN, Zakład Andrologii Molekularnej, ul. Tuwima 10, 10-747 Olsztyn. Tel. +48 89 5357429, e-mail: bcejko@poczta.onet.pl