Bożena Szczepkowska, Andrzej K. Siwicki, Mirosław Szczepkowski, Edward Głąbski, Barbara Kazuń, Krzysztof Kazuń, Elżbieta Terech-Majewska, Elżbieta Majewicz-Zbikowska
ABSTRACT. The aim of the study was to determine the impact of different doses of 1,3-1,6-b-D glucan (Leiber-Beta S, Germany) on the results of rearing and the non-specific cellular and humoral defense mechanisms in whitefish reared in a closed system. The study material comprised fry with a mean body weight of 21.2 g and a mean body length of 12.4 cm. The experiment was 129 days long. Three feeding treatments were applied: control group (K) - standard feed without the addition of glucan; group G-200 with the addition of Leiber-Beta S glucan at a dose of 200 g per ton of feed; group G-500 with the addition of Leiber-Beta S glucan at a dose of 500 g per ton of feed. No significant differences were noted among the treatment groups with regard to growth or feed conversion ratio. In comparison to the control group, statistically significant increases in macrophage phagocytic activity and in T and B lymphocyte proliferation were noted in the fish fed dietary treatments with the addition of glucan. Simultaneously, statistically significant increases in lyzozyme activity and in serum Ig levels were noted in comparison with those in the control groups. However, no significant differences were noted between the doses of 200 and 500 g of glucan per ton of feed. The results of this study indicate that the application of Leiber-Beta S glucan stimulates the non-specific defense mechanisms in whitefish at doses of 200 and 500 g per ton of feed.
Keywords: whitefish, defense mechanisms, glucan, closed systems
Przyjęto po recenzji 19.11.2009 r.