Andrzej K. Siwicki, Barbara Kazuń, Krzysztof Kazuń, Edward Głąbski, Ewa Szczucińska, ElżbietaTerech-Majewska
ABSTRACT. The aim of the study was to determine the influence of infection induced by Flavobacterium branchiophilum on the nonspecific cell-mediated and humoral-mediated immunity in rainbow trout fingerlings. Rainbow trout (20 g body weight) with dystrophic changes in the gills were examined. Flavobacterium branchiophilum was isolated from the gills and bacterial gill disease (BGD) syndrome was observed. When the first symptoms of BGD appeared, blood from 20 diseased and 20 healthy fish was drawn to examine the respiratory burst activity (RBA) and potential killing activity (PKA) of blood phagocytes. The lysozyme activity and total protein and gammaglobulin (Ig) levels were also examined. A strong immunosuppressive effect was observed in fish with BGD during the present immunological studies, and all immunological parameters were statistically significantly lower in fish with BGD syndrome in comparison to those of healthy fish. The preliminary study indicated that BGD has a strong suppressive effect on blood phagocyte activity and nonspecific humoral-mediated immunity.
Keywords: rainbow trout, Flavobacterium branchiophilum, cell-mediated and humoral-mediated immunity
Przyjęto po recenzji 24.11.2010 r.