Beata Sarosiek, Beata I. Cejko, Sławomir Krejszeff, Jan Glogowski, Katarzyna Targońska, Dariusz Kucharczyk, Daniel Żarski, Radosław K. Kowalski
ABSTRACT. One of the ways to improve methods for the artificial reproduction of fish is the possibility of storing sperm for short periods under refrigerated conditions (+4oC) without the necessity of freezing. In the experiment semen extenders with and without sugars (glucose and fructose) were applied during the storage of pike semen. The analysis of sperm motility parameters indicated that semen that had not been diluted with an immobilization buffer decreased in quality very quickly. After 24 hours of storage significant differences were noted in parameters measured with CASA, and after the subsequent two days no motile sperm were noted. The semen that was diluted with the immobilization buffer that included glucose retained 48% motile sperm after four days of the experiment. The results indicate that diluting the semen with an immobilization buffer lengthens the storage period of pike semen under refrigerated conditions.
Keywords: pike, semen, sperm, storage, CASA
Przyjęto po recenzji 03.02.2012 r.