ABartosz Bojarski, Leszek Szała, Bartłomiej Stonawski, Michał Inglot, Czesława Gaj-Chucher
ABSTRACT. Hematological analysis is used, among others, to assess fish health and welfare. The routinely analyzed hematological parameters include hematocrit, i.e. the ratio of the volume of erythrocytes to the volume of whole blood. In the case of fish blood, the most common method of hematocrit determination is the microhematocrit method, which is based on centrifuging the blood in a capillary tubes and reading the hematocrit with a hematocrit reader. Centrifugation is carried out for a specified time and at a specified relative centrifugal force (RCF) value. The aim of this study was to determine how the relative centrifugal force influences the obtained hematocrit values. For this purpose, 20 samples of rainbow trout (Oncorhynchus mykiss) blood were centrifuged for 5 minutes with 16,000 g, 13,000 g, 10,000 g, 7,000 g and 4,000 g. The obtained data was analyzed by one-way analysis of variance for repeated measurements. In each case, the reduction of the RCF value resulted in a statistically significant increase in the hematocrit value. The obtained results indicate the necessity to develop standard, commonly accepted parameters of fish blood samples centrifugation in the microhematocrit method.
Keywords: hematocrit, centrifugation, methodology, hematology
Przyjęto po recenzji 3.10.2022 r.